Grid preparation, imaging, data acquisition, and structural analysis technology for membrane protein analysis (Patents US.11,994,525, JP.7046409, CN116194781, KR.102588805, AU.2021372121)
Cryo-LSEM is a grid preparation, imaging, data acquisition, and structural analysis technology for cryo-electron microscopy that directly forms lipid bilayers in the pores of carbon support membranes on electron microscopy grids, enabling membrane proteins to be maintained in a physiologically relevant environment within the lipid bilayer for imaging and structural analysis.
Structural analysis of membrane proteins by cryo-electron microscopy has faced challenges, particularly regarding the need for high-concentration, high-purity samples. This technology uses a proprietary protocol to form lipid bilayers in a planar configuration on grids, allowing efficient concentration of membrane proteins and data acquisition even from small-volume, low-concentration samples. Three-dimensional reconstruction is expected to be possible for difficult samples that would not be amenable to existing techniques, such as samples with low yields that can only undergo affinity purification and are difficult to concentrate or prepare as high-purity samples, as well as unstable protein complex samples. Using this technology, we have successfully achieved three-dimensional reconstruction from affinity-purified samples of unstable membrane protein complexes that could only be concentrated to approximately 0.03 mg/ml. Although the number of implementation examples is currently limited, based on the principles of this technology, we believe that data acquisition with membrane proteins embedded in lipid bilayers should be possible for a wide range of membrane proteins, provided they can be embedded in liposomes.
Please note that this technology targets membrane proteins that function embedded in membranes and is not applicable to soluble proteins.
The membrane protein samples targeted by this technology are those solubilized with detergents. Please note that membrane protein samples already embedded in lipid bilayers, such as those in liposomes or nanodiscs, are not considered suitable for grid preparation based on the principles of this technology.
For license set sales, we will confirm the shipping address for the Teflon instruments, provide a link to the protocol when production of the Teflon instruments becomes possible, issue a serial number, and process payment. After production of the Teflon instruments begins, refunds and cancellations are not available except in cases where the Teflon instruments fail to arrive due to our responsibility. Please acknowledge this condition.
If customs duties or surcharges are incurred in the recipient's country of residence during transport of the Teflon instruments, the recipient must complete the payment procedures themselves.
The license set includes one grid preparation experiment and technical licenses for data acquisition and data analysis using the prepared grids. If additional grid preparation experiments are to be conducted, please purchase the license set again. Up to 8 grids can be prepared in a single grid preparation experiment, allowing for evaluation of up to 8 conditions.
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Individual sales of Teflon instruments or manuals are not available.
| Prohibited Activities | Redistribution, resale, and sublicensing are prohibited |
| License Period |
One year for grid preparation experiments and acquisition of primary data. Rights to acquired data belong to the customer in principle. |
| Taxes | Tax included |
| Payment Method | Credit card |
| Refund and Cancellation Policy | No refunds or cancellations after purchase |
| Support | Individual support provided based on serial number |
| Version Updates and Changes | Announcements will be made on the website |
| Disclaimer of Liability | We assume no responsibility for any damages resulting from the use of services or products. |
| Governing Law and Jurisdiction | This agreement is governed by Japanese law, and the Fukuoka District Court or Fukuoka Summary Court shall be the exclusive agreed jurisdiction. |