Proprietary Technology Enabling Structural Biology of Challenging Membrane Proteins

High-Resolution Imaging and Structural Analysis of Membrane Proteins

Lipid bilayer-Supported specimen Electron Microscopy (LSEM), a patented technology developed through the founder's invention, enables simple high-resolution imaging of membrane proteins within lipid bilayers. Additionally, "cryo-LSEM," a technique combining LSEM with cryo-electron microscopy (cryo-EM), makes 3D reconstruction possible at low concentrations around 0.03 mg/ml, which is difficult to achieve with existing methods.

Our technology is a proprietary technique that enables simple, high-resolution imaging and structural analysis of membrane proteins within lipid bilayers.

Purchase License Set

Reduced Required Sample Purity

Even with low-purity samples that have limited yield and can only be purified up to the affinity chromatography stage, the membrane proteins are further purified when embedded in the lipid bilayer within the grid, enabling 3D reconstruction through single particle analysis (SPA) in cryo-LSEM.

Reduction of Required Sample Concentration and Volume

Because membrane proteins are concentrated in the lipid bilayer, 3D reconstruction by cryo-LSEM is possible even with low-concentration and small-amount samples.

Expanded Analysis Scope

Cryo-LSEM can handle structural analysis of large membrane protein complexes that were difficult to analyze with nanodiscs.

2026.08.30

U.S. Patent Registration Notice

2024.05.20

Our Technology

Cryo-LSEM License Set

$1,800.00

【セット内容】
・cryo-LSEM technology license (serial number) ×1
・Manual ×1
・Teflon instrument (single use) ×1

Technology license for membrane protein analysis grid preparation, imaging, data acquisition, and structural analysis (Patents US.11,994,525, JP.7046409, CN.116194781, KR.102588805, AU.2021372121)

Cryo-LSEM is a cryo-electron microscopy grid preparation, imaging, data acquisition, and structural analysis technology that directly forms lipid bilayers in the pores of carbon support membranes on electron microscopy grids, allowing membrane proteins to be maintained and data acquired in a physiologically relevant environment within the lipid bilayer.

We are now offering a limited advance pre-order of 20 technology license sets for cryo-LSEM. Future sales will be announced on our website. Please note that due to the nature of this product, we anticipate sales to customers affiliated with corporations, public research institutions, universities, and other organizations for business purposes. Sales for personal use are not intended. The rights to all data acquired using this license set shall, in principle, belong entirely to the customer. However, when publishing data externally through academic papers or other means, please display our company name in the Acknowledgements section to indicate that the data was acquired under an appropriate license. Additionally, grid preparation experiments and primary data acquisition must be completed within one year of receiving the license set.

About cryo-LSEM

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